中国食品学报2011，Vol.11Issue(6)：42-46,5.
雷丸多糖的提取分离及其抗氧化活性研究
Study on Extraction, Isolation and Antioxidant Activity of Polysaccharide from Omphalia Lapidescens
摘要
Abstract
Objective:To investigate the extraction and purification of Omphalia lapidescens polysaccharide and its an-tioxidant activities. Methods: The 0. Lapidescens polysaccharide was extracted after enzymolysis by cellulase, through alcohol fractional precipitation,free proteins and colors were removed by Sevage method and charcoal, respectively. 0L1, OL2 and 0L3 were isolated and purified by Sephadex G-200 column. The best condition for enzymatic hydrolysis of 0. Lapidescens polysaccharide was studied:adding amount of cellulase 0.08%, enzymatic hydrolysis for 90 min at 35 °C. Results: The extraction time was reduced greatly by cellulase enzymatic hydrolysis method, which indicates that it is a potential method for extraction polysacchatides from 0. Lapidescens. Omphalia polysaccharide is a kind of good source for natural antioxidants, has relatively strong antioxidant activities and worth further development and utilization.关键词
雷丸/提取/分离/抗氧化活性Key words
Omphalia lapidescens/ extraction/ isolation/ antioxidant activity引用本文复制引用
许明峰,沈莲清,王奎武,王向阳..雷丸多糖的提取分离及其抗氧化活性研究[J].中国食品学报,2011,11(6):42-46,5.基金项目
浙江省科技厅研究生创新科研项目(YK2008063) （YK2008063）
