中国食品学报2011，Vol.11Issue(6)：152-157,6.
多重PCR同时检测常见8种食物过敏原
Detection of Eight Food Allergens with Multiplex PCR
摘要
Abstract
Eight pairs of primers were selected according to the sequence of Lectin gene from soybean, Gliadin gene from wheat, Ara h 3 gene from peanut, Ana o3 gene from cashew, 16S rRNA from shrimp and fish, mitochondrial DNA from bovine and chicken respectively, and a multiplex PCR assay for food allergen detection was established on the basis of simple PCR technique. Eight food allergens could be detected rapid, specific and sensitive with 2 multiplex PCR assays at the same time. It could be used for detection and surveillance of many allergens in food.关键词
多重PCR/食物过敏原/检测Key words
multiplex PCR/ food allergens/ detection引用本文复制引用
王玮,韩建勋,袁飞,吴亚君,陈颖,葛毅强..多重PCR同时检测常见8种食物过敏原[J].中国食品学报,2011,11(6):152-157,6.基金项目
国家自然科学基金项目(No.31071552) （No.31071552）
国家“十一五”科技支撑计划项目(No.2009BADB9B03) （No.2009BADB9B03）
