郑州轻工业学院学报（自然科学版）2011，Vol.26Issue(4)：22-26,5.
柽柳核纤孔菌分子鉴定及基于ITS序列的系统发育分析
Molecular characterization of lnocutis tamaricis and phylogenetic analysis of related taxa using ITS sequence analysis
摘要
Abstract
Inocutis tamaricis 6 - 27, collected in Zhengzhou of He'nan province, China, was identified primarily based on morphological characteristics. The rDNA ITS region of the strain was cloned and se-quenced,and BLAST matched with GenBank database on homology. The ITS sequences,together with 22 reference sequences obtained from the GenBank database, were used to construct a phylogenetic tree. The results demonstrated that the ITS sequence analysis supported the identification based on morphological characteristics, the strain was Inocutis tamaricis. In phylogenetic analysis, twenty three materials from different genera were divided into six groups. Group A included seven strains of /. tamaricis and one strain of In-onotus cuticularis. I. jamaicensis 4508 from southern hemisphere from Argentina and Fomitiporella caryo-phylli CBS 448. 76 from India were integrated into group B,as big sister group with group A and small sister group with group C, which included two Fulvifomes species. The remained nine strains of Inonotus, Phellinus,Mensularia and Fuscoporia were integrated into group D,E and F. The phylogenetic analysis of allied taxa suggested the independent genus level of Inocutis and Fulvifomes, however, the heterogeneous status of genera Inonotus and Phellinus needs further standardizing and unifying.关键词
ITS序列分析/分类鉴定/系统发育/刺革菌目/大型真菌Key words
ITS sequence analysis/ classification and identification/ phylogeny/ Hymenochaetales/ macro-fungi分类
生物学引用本文复制引用
魏涛,何培新,刘伟,耿卢婧..柽柳核纤孔菌分子鉴定及基于ITS序列的系统发育分析[J].郑州轻工业学院学报（自然科学版）,2011,26(4):22-26,5.基金项目
河南省重点科技攻关项目(102102210063) （102102210063）
郑州轻工业学院博士基金项目(000544) （000544）
