中国食品学报2011，Vol.11Issue(7)：133-139,7.
绿色木霉菌抑菌培养基优化研究
Studies on Optimization of Medium for Bacteriostasis by Trichoderma Viride
摘要
Abstract
Response surface methodology (RSM) was used to optimize the medium for bacteriostasis by Trichoderma Viride Pers. ex Fr. With several plant pathogens as the tested strains, the inhibitory rate was determined by using confront culture method. According to the single-factor experiments, three factors (glucose addition, alanine addition and KH2PO4 addition) were selected for the central composite designed to establish a quadric regression equation. RSM was employed to optimize. The results showed that the best carbon source, nitrogen source, phosphorus source and their optimum content were glucose 2.18%, alanine 0.15% and KH2PO4 0.3%. Under these conditions, the inhibitory rate of T. Viride against Colletotrichum magiferae P. Henn., Colletotrichum gfoeosporioides Penz. and Fusarium oxysporum Schlecht. reached to 82.29%, 78.43% and 71.63% respectively. Therefore, T. Viride could inhibit the growth of several plant pathogens significantly. And medium have influence on its bacteriostatic ability.关键词
绿色木霉/培养基/植物病原菌/抑菌作用Key words
Trichoderma viride/ medium/ plant pathogen/ bacteriostasis引用本文复制引用
黄雪莲,于新..绿色木霉菌抑菌培养基优化研究[J].中国食品学报,2011,11(7):133-139,7.基金项目
国家自然科学基金资助项目(30871765) （30871765）
