中国食品学报2011，Vol.11Issue(7)：89-94,6.
响应曲面法优化黑莓叶中总黄酮的提取工艺
Optimization of Extraction Technology of Total Flavonoids from Blackberry Leaves using Response Surface Methodology
摘要
Abstract
Purpose:In this paper, designs of experiments were used to screen the significant factors that effect the extraction of total flavonoids in blackberry leaves, and optimize extraction conditions. Methods: Based on the results of single factor experiment, factorial design was used to screen the significant factors. Mathematical model of independent variable was established and the levels of these significant factors were determined by central composite design and response surface methodology. Results; Ultrasonic power, extraction time and solvent-to-material ratio had significant influences on the responses. The optimized extraction conditions were:ultrasonic power 757.8 W, extraction time 5.8 min and solvent-to-material ratio 118.1. Under the optimized conditions, the total flavonoid yield in blackberry leaves reached to 21.96mg/g. Conclusion; Regression model established in the study can predict the extraction of total flavonoids in blackberry leaves commendably.关键词
黑莓叶/黄酮/微波提取/关键因子/响应曲面法Key words
blackberry leaves/ flavonoid/ microwave extraction/ significant factor/ response surface methodology引用本文复制引用
刘小莉,周剑忠,朱佳娜,孟璐..响应曲面法优化黑莓叶中总黄酮的提取工艺[J].中国食品学报,2011,11(7):89-94,6.基金项目
江苏省科技支撑项目(BE2009362) （BE2009362）
