中国食品学报2011，Vol.11Issue(7)：95-101,7.
酶解法制备低蛋白含量海参多糖条件的研究
Study on Preparation Conditions of Sea Cucumber Polysaccharide with Low Protein by Enzymolysis
摘要
Abstract
In this research, using general low-value sea cucumber as raw material, enzymolysis was combined with ultrafiltration to prepare low-protein sea cucumber polysaccharide. Response surface methodology was adopted to study and optimize the parameters affecting protein content on the basis of single factor experiments. A second order quadratic e-quation was established. Results indicated that the optimal levels for achieving polysaccharide with the lowest protein content were obtained, including pH7.0, temperature of 51℃, substrate concentration of 4.82%, the ratio of enzyme to substrate of 4.08% and time of 12 h. The protein content of polysaccharide was 2.41%. Therefore, it could provide theoretical guidance for industrialized preparing sea cucumber polysaccharide with low protein. And it would benefit deep processing of low-value sea cucumber.关键词
海参多糖/糖胺聚糖/蛋白含量/酶解/响应面Key words
sea cucumber polysaccharide/ glycosaminoglycan/ protein content/ enzymolysis/ response surface methodology引用本文复制引用
宿玮,常耀光,张翠玉,崔宏博,薛长湖..酶解法制备低蛋白含量海参多糖条件的研究[J].中国食品学报,2011,11(7):95-101,7.基金项目
海洋公益性行业科研专项(No.201105029) （No.201105029）
国际科技合作项目(No.2010DFA31330) （No.2010DFA31330）
中央高校基本科研业务费专项资金资助项目 （）
