中国食品学报2011，Vol.11Issue(7)：110-117,8.
响应面法优化副干酪乳杆菌HD1.7产Paracin1.7发酵培养基
Optimization of Medium for AMP Production from Lactobacillus Paracasei HD1.7 by Response Suface Methodology
摘要
Abstract
Objective:To study the medium optimization of Antimicrobial peptides (Paracinl.7) production from Lacto-bacillus paracasei HD1.7. Methods:A Plackett-Burman design was first used to sift the important factors from eight relat-ed faetors. Then, the path of steepest aseent and the Box-Behnken design were used for further optimization. By JMP 7.01 solving the quadratic regression model equation. Results: Glueose、MgSO4、 MnSO, were affirmed the important fae-tors in the medium by PB design,and the optimal concentrations of the variables were determined as:glueose 6.5685 g/L, MgSO4 0.454 g/L,MnSO4 0.01052 g/L. Under the optimal culture condition, the titre of the AMP was up to 141.648 IU/ mL.this was improved more than 1.91 fold befor optimal. Conclusion: The AMP produced by Lparacasei HD1.7 can inhibit not only G+, but also G- and yeast.it can be used as a new type of food preservative, this study lay the founda-tion for future industrial production of Antimicrobial peptides (Paracinl.7).关键词
响应面法/副干酪乳杆菌HD1.7/抗菌肽/优化Key words
response surfaee method/ Lactobacillus paracaseiHD1.7/ antimicrobial peptides (AMP)/ optimization引用本文复制引用
葛菁萍,由田,高先军,苑婷婷,孙红兵,平文祥..响应面法优化副干酪乳杆菌HD1.7产Paracin1.7发酵培养基[J].中国食品学报,2011,11(7):110-117,8.基金项目
黑龙江省科技攻关项目(No.GB05 B401) （No.GB05 B401）
