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清香型乌龙茶品质形成过程中儿茶素类和嘌呤碱指纹图谱变化规律

陈林 陈键 张应根 邬龄盛 王振康 杨卫 尤志明

茶叶科学2011,Vol.31Issue(6):493-503,11.
茶叶科学2011,Vol.31Issue(6):493-503,11.

清香型乌龙茶品质形成过程中儿茶素类和嘌呤碱指纹图谱变化规律

Variation of Catechins and Purine Alkaloids Fingerprints during the Formation of Fresh Scent-Flavor Oolong Tea

陈林 1陈键 1张应根 1邬龄盛 2王振康 1杨卫 1尤志明2

作者信息

  • 1. 福建省农业科学院茶叶研究所,福建福安355015
  • 2. 安徽农业大学农业部茶及药用植物安全生产重点开放实验室,安徽合肥230036
  • 折叠

摘要

Abstract

The spring shoots of Tieguanyin, Huangdan, Jinguanyin, Huangguanyin (Camellia sinensis) was respectively used as materials for the test, and catechins and purine alkaloids of the samples prepared during the period of shoot growth and the process of fresh scent-flavor Oolong tea were determined by high-performance liquid chromatography. The results showed that the stability, precision and reproducibility of the method were qualified for requirements to build catechins and purine alkaloids fingerprints of tea samples. When dealt with ChemPattern 2.0 Professional Version, a kind of fingerprint data processing software, the consistent pattern recognition results could be obtained by similarity analysis, cluster analysis and principal component analysis. The fingerprints of various tea samples were quite different during the period of shoot growth, and could be distinguished from the WIP of fresh scent-flavor Oolong tea. Every tea sample of the WIP of fresh scent-flavor Oolong tea could be well identified by cluster analysis (Euclidean-NearestNeighbor), especially for principal component analysis (data pretreated with Log Transformation). The change in the content of (-)-epigallocatechin (EGC) was the most obvious during the period of shoot growth in each tea sample, which was followed by (-)-epigallocatechin gallate (EGCG) and caffeine. While the change in the content of (-)-epigallocatechin gallate (EGCG) was more obvious during the process of fresh scent-flavor Oolong tea, which was followed by EGC. The contents and compositions of catechins and purine alkaloids changed more significantly during the period of shoot growth than those in the process of fen-flavor Oolong tea.

关键词

乌龙茶/指纹图谱/儿茶素/嘌呤碱

Key words

Oolong tea, fingerprint, catechins, purine alkaloids

分类

轻工纺织

引用本文复制引用

陈林,陈键,张应根,邬龄盛,王振康,杨卫,尤志明..清香型乌龙茶品质形成过程中儿茶素类和嘌呤碱指纹图谱变化规律[J].茶叶科学,2011,31(6):493-503,11.

基金项目

福建省属公益类项目(2009R10030-1)、国家茶叶产业技术体系建设专项 (2009R10030-1)

茶叶科学

OA北大核心CSCDCSTPCD

1000-369X

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