磷酸盐缓冲液的浓度和pH对革胡子鲶肠道抗菌蛋白/肽分级盐析的影响OA
Effect of Concentration and pH Value of Phosphate Buffer on Gradient Ammonium Sulphate Fractionation of Antibacterial Protein/peptide from Intestine of African Catfish ( Clarias gariepinus)
[目的]探讨磷酸盐缓冲液的pH和浓度在分级盐析时,对革胡子鲶肠道各组分蛋白/肽的影响.[方法]以不同浓度和pH的磷酸盐缓冲液作为组织匀浆缓冲液,在硫酸铵饱和度为20％ 、40％、60％、80％和100％的条件下,对革胡子鲶肠道抗菌蛋白/肽进行分级盐析,并对盐析产物进行称重、电泳检测和抑菌活性分析.[结果]磷酸盐缓冲液的pH为6.0或7.4时,其浓度对盐析产物产量、蛋白沉淀和抑菌效果无显著影响.而pH为8.0、浓度为0.05 mol/L时,硫酸铵饱…查看全部>>
[Objective] The effects of the concentration and pH value of phosphate buffer on the proteins/peptides in each fraction of African catfish in testine were studied. [ Method] The antibacterial proteins/peptides from intestine of African catfish were fractionation by a graded ammonium sulfale (AS) precipitation technique and the fractions were oblained at 20%,40%,60%,80% and 100% saturation with ammo nium sulfale. Each fraction was weight and detected by SDS-P…查看全部>>
王存;王晓梅;季延滨;徐敏;戴伟;潘宝平
天津师范大学生命科学学院,天津300387天津市水产生态及养殖重点实验室,天津农学院水产科学系,天津300384天津市水产生态及养殖重点实验室,天津农学院水产科学系,天津300384天津市水产生态及养殖重点实验室,天津农学院水产科学系,天津300384天津师范大学生命科学学院,天津300387天津市水产生态及养殖重点实验室,天津农学院水产科学系,天津300384
农业科技
革胡子鲶肠道抗菌肽磷酸盐缓冲液
African catfish Intestine Antibacterial protein/peptide Phosphate buffer
《安徽农业科学》 2011 (28)
17344-17346,17394,4
天津市应用基础及前沿技术研究计划重点研究项目(08JCZDJC19000).
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