北京工商大学学报：自然科学版2011，Vol.29Issue(6)：32-34,39,4.
鸡精中呈味核苷酸二钠的高效液相色谱法测定
Quantitative Determination of Disodium 5′-Inosinate and Disodium 5′-Guanylate in Chicken Extract by HPLC
相丽新 1杨道兴 1魏彦梅 1颜丙霞1
作者信息
- 1. 济宁耐特食品有限公司,山东济宁272075
- 折叠
摘要
Abstract
A high performance liquid chromatography method was established for quantitative determination of disodium 5′-inosinate and disodium 5′-guanylate（IMP and GMP）.Using tetrabutylammonium phosphate monobasic and potassim dihydrogen phosphate mixed with a certain proportion of acetonitrile as the mobile phase,the standard samples were analyzed on Waters RP 18 column under the optimal conditions.The qualitative and quantitative reproducibility of RSD were below 2%.On the IMP standard curve,the linear range was 60~600 μg/mL,the correlation coefficient R being 1.000.On the GMP standard curve,the linear range was 50~480 μg/mL,the correlation coefficient R being 0.999 9.The method was used to determine IMP and GMP content in a certain brand of chicken extract,which was 0.54% and 0.60%,respectively,the precision being 0.73% for GMP,1.17% for IMP.关键词
高效液相色谱/呈味核苷酸二钠/定性定量重复性/相对标准偏差/鸡精Key words
high performance liquid chromatography/disodium 5′-inosinate and diosidm 5′-guanylate/qualitative and quantitative reproducibility/RSD/chicken extract分类
轻工纺织引用本文复制引用
相丽新,杨道兴,魏彦梅,颜丙霞..鸡精中呈味核苷酸二钠的高效液相色谱法测定[J].北京工商大学学报：自然科学版,2011,29(6):32-34,39,4.
