茶叶科学2012，Vol.32Issue(1)：37-43,7.
利用固定化纤维素酶酶解夏季绿茶工艺的研究
Study on the Influence of Immobilized Cellulase on the Summer Tea Extract
摘要
Abstract
Immobilization of cellulase on carrier of chitosan beeds crosslinked by glutaraldehyde were studied, and applied the immobilized cellulase to the hydrolysis of the summer green tea. Through the orthogonal test, the optimal extract technology has been investigated: adding 1.0 g immobilized cellulose enzyme in 1.0 g green tea, the ratio of tea and water is 1 : 20, and extracted 50min under 55 ℃. Compared to the traditional tea extraction method by hot water, the contents of tea polyphenols, amino acid and caffeine increased by using this technology, and the ratio of polyphenol to ammo acid was decreased. The immobilized cellulase still maintain a high activity, the relative activity of enzyme was more than 80%, after 7 times extraction.关键词
壳聚糖/纤维素酶/固定化酶/夏季茶叶Key words
chitosan/ cellulose/ immobilized enzyme/ summer tea分类
轻工纺织引用本文复制引用
汪珈慧,李燕,姚紫涵,王家健..利用固定化纤维素酶酶解夏季绿茶工艺的研究[J].茶叶科学,2012,32(1):37-43,7.基金项目
骆肇荛科创基金项目第五期 （）
