现代食品科技2011，Vol.27Issue(10)：1217-1220,1209,5.
交联β-葡萄糖苷酶聚集体的制备及应用
Preparation and Application of Cross-Linkedβ-Glucosidase Aggregates
摘要
Abstract
Cross-linked enzyme aggregates (CLEAs) technique is a novel carrier-free immobilization method. In the present work, β-glucosidase from prune (Prunus domestica) seeds was immobilized by this method. The preparation conditions of CLEAs were optimized, and the synthesis of propyl β-D-glucopyranoside catalyzed by this immobilized enzyme was investigated. The optimum precipitant and its concentration, cross-linking agent (glutaraldehyde) concentration, cross-linking time were n-propanol, 2/1 (V/V), 10 mmol/L and 2 h, respectively. Under the optimum conditions, the activity recovery of n-propanol-CLEAs reached 84%. In the synthesis of propyl β-D-glucopyranoside catalyzed by the immobilized enzyme, the yield of the desired product was 60% after 96 h.关键词
西梅籽/交联酶聚合物(CLEAs)/固定化/β-葡萄糖苷酶Key words
Prunus domestica seeds/cross-linked enzyme aggregates (CLEAs)/immobilization/β-glucosidase引用本文复制引用
陈磊,李宁,宗敏华..交联β-葡萄糖苷酶聚集体的制备及应用[J].现代食品科技,2011,27(10):1217-1220,1209,5.基金项目
华南理工大学制浆造纸工程国家重点实验开放基金项目(200913) （200913）
