现代食品科技2011，Vol.27Issue(10)：1238-1241,4.
耐高温植酸酶毕赤酵母工程菌发酵中试条件优化
Optimization of Pilot-Scale Conditions of Thermostable Phytase Fermentation by Engineered Pichia pastoris Strain
黄魁英 1夏枫耿 1黄乐天 1明飞平 1杜少平1
作者信息
- 1. 广州市微生物研究所,广东广州510663
- 折叠
摘要
Abstract
The phytase fermentation conditions of the genetic-engineered Pichia pastoris strain PEY-2 were studies in this paper. The optimal phytase fermentation conditions were: induction time 72 h, enrichment time before induction 48 h, inoculum size 10%, seed age 24 h, pH for cell growth and inducement at 5.5 and 6.0 respectively. In scale-up culture in 50 L fermenter, phytase fermentation of high density is achieved and the activity of phytase highly reached 5.0×103 IU/mL by constant dissolve oxygen fed-batch way of glucose. Thermal stability tests showed that after 20-minute incubation at 90℃, the remaining activity of the phytase was 69% at pH 2.5 and 75% at pH 5.5 respectively,. The phytase was thermostable and could meet requirements of feed processing.关键词
植酸酶/毕赤酵母/发酵中试/优化/热稳定性Key words
phytase/pichia pastoris/pilot-scale fermentation/optimization/Heat stability引用本文复制引用
黄魁英,夏枫耿,黄乐天,明飞平,杜少平..耐高温植酸酶毕赤酵母工程菌发酵中试条件优化[J].现代食品科技,2011,27(10):1238-1241,4.
