现代食品科技2011，Vol.27Issue(10)：1264-1267,4.
应用LAMP实时浊度法检测转基因大豆
Development of a Real-time Turbidimeter-based Loop-mediated Isothermal Amplification Assay for Detection of Transgenic Soybean
摘要
Abstract
Loop-mediated isothermal amplification method (LAMP) is a novel nucleic acid ampli- fication technology. The LAMP method amplifies DNA with rapidity, high specificity and sensitivity under isothermal conditions. Since turbidity of the reaction mixture would increase in correlation with the DNA yield, real-time monitoring of the LAMP reaction was achieved by real-time turbidimeter. Enolpyruvl Shimimate phosphate syntheses gene was amplified by a set of four specially primers that recognize six distinct sequences of the target. The amplification can be obtained in 1 h by incubating all of the reagents in a single tube by real-time turbidimeter at 63 ℃. Results from this study showed that the LAMP method was an effective method for the rapid detection of Transgenic Soybean and their test results were consistent with the results of conventional PCR methods. LAMP assay results were found to be 10 times more sensitive than the conventional PCR. The LAMP detection method was specific, stable and reliable, and will be an effective tool for rapid detection of Transgenic Soybean.关键词
环介导等温核酸扩增技术/实时浊度仪/转基因大豆/检测Key words
LAMP/real-time turbidimeter/transgenic soybean/detection引用本文复制引用
袁瑛娜,单潇潇,王宗德,石磊,袁秀金,谭贵良..应用LAMP实时浊度法检测转基因大豆[J].现代食品科技,2011,27(10):1264-1267,4.基金项目
广东省质监局科技计划项目(2009zz11) （2009zz11）
