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基于流感A&B核蛋白的高灵敏度免疫层析的快速诊断方法的建立及临床应用

刘晓云 杨连生 王继华 彭运平 冯红 巫宏伟 张建 李国存 李辉

现代食品科技2011,Vol.27Issue(11):1421-1424,4.
现代食品科技2011,Vol.27Issue(11):1421-1424,4.

基于流感A&B核蛋白的高灵敏度免疫层析的快速诊断方法的建立及临床应用

The Development and Application of High Sensitivity Influenza A&B Fast Test Strip on Patients with Fever

刘晓云 1杨连生 2王继华 1彭运平 2冯红 2巫宏伟 2张建 2李国存 2李辉2

作者信息

  • 1. 华南理工大学轻工与食品学院,广州广东510640
  • 2. 广州万孚生物技术有限公司,广州广东510663
  • 折叠

摘要

Abstract

A double antibody sandwich immunachromatopgraphic fast test method was established by choosing high specific influenza A &B nucleoprotein, and its sensitivity and specific were investigated And then fever contributed pharyngeal swabs of 1928 patients were tested by this method and the Consistency for Influenza A and B was compared with that by commercial GICA assay. The sensitivity for influenza A&B was diluted to 1:5000 for TC1D50, and no cross-reaction was found with other common virus. Positive coincidence rate for influenza A were 95.75% and 92.52%, Negative coincidence rate for influenza B were 92.95% and 99.53%, respectively. This fast test method showed high sensitivity and specificity, which had the great potential in the management of infuluenza A&B disease on patients with fever.

关键词

流感A/流感B/快速检测

Key words

influenza A/ influenza B/ fast test

引用本文复制引用

刘晓云,杨连生,王继华,彭运平,冯红,巫宏伟,张建,李国存,李辉..基于流感A&B核蛋白的高灵敏度免疫层析的快速诊断方法的建立及临床应用[J].现代食品科技,2011,27(11):1421-1424,4.

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