现代食品科技2012，Vol.28Issue(2)：164-167,155,5.
酶解菲牛蛭制备抗凝血肽的工艺优化
Optimization of Enzymatic Hydrolysis ofPoecilobdella manillensis Lesson for Preparing ofAncoagulant Peptide
摘要
Abstract
In order to obtain high activity ancoagulant peptide from Poecilobdella manillensis Lesson, an enzymolysis method was established through sequential steps of screening single-factor and designing orthogonal test. By selecting ancoagulant activity as index, the optimum enzymolysis reaction conditions were determined as enzymolysis temperature 55 °C, enzymolysis temperature 2h, substrate concentration 12%, enzyme dosage 3% and pH8.5. The ancoagulant activity could reach 702AT-U/g under the optimized conditions.关键词
菲牛蛭/抗凝血活性/Alcalase 2.4 L/抗凝血肽Key words
Poecilobdella manillensis Lesson/ ancoagulant activity/ Alcalase 2.4L/ ancoagulant peptide引用本文复制引用
钟超,吴晖,赖富饶..酶解菲牛蛭制备抗凝血肽的工艺优化[J].现代食品科技,2012,28(2):164-167,155,5.基金项目
新世纪优秀人才支持计划项目(NCET-060746) （NCET-060746）
