肉类研究Issue(11)：1-5,5.
猪肝脏蛋白质组双向电泳体系的建立及优化
Proteome Analysis of Pork Liver by Two-Dimensional Gel Electrophoresis
摘要
Abstract
In the present study, a two dimensional （2-DE） gel electrophoresis system was established and optimized for pork liver proteome. The sample preparation was achieved using ultracentrifugation and cleanup kits and ready IPG strips ImmobilineTM DryStrip gel （ 17 cm and pH5 - 8） were used at a sample loading level of 120/a L, resulting in a matching rate of 76% and 1412 ± 8 protein spots. According to the total blindness method, accuracy and repeatability tests were performed on different pork liver samples, showing a matching rate of over 70% and spots having a relative mobility within the laboratory control standards. Therefore, protein spots having statistical significance and allowing quantitative analysis could be screened out and be used for the differentiation of pork liver samples. In conclusion, the established system can allow 2-DE gel electrophoresis of sample pork liver samples.关键词
猪/肝脏/蛋白质组/双向电泳/优化Key words
pork/liver/proteome/two dimensional gel electrophoresis （2-DE）/optimization分类
轻工纺织引用本文复制引用
王新洁,韩剑众,曲道峰..猪肝脏蛋白质组双向电泳体系的建立及优化[J].肉类研究,2011,(11):1-5,5.基金项目
浙江省重大科技专项 （）
