肉类研究Issue(11)：29-33,5.
环介导恒温扩增法检测肉制品中致病微生物
Rapid Detection of Listeria monocytogenes in Meat Product Using Loop-Mediated Isothermal Amplification Method
陈洵 1冯雪梅 1杜正平 1谭慧媚 1田文武 1廖秀志 1曹以诚1
作者信息
- 1. 广州华峰生物科技有限公司,广东广州510663
- 折叠
摘要
Abstract
Objectives： To establish a method for rapid detection of microbial nucleic acids in food, develop a convenient, rapid and sensitive nucleic acid detection kit, and apply the kit for Listeria monocytogenes detection in meat product. Methods： According to the sequence of the characteristic target gene vir R, specific primer pairs were designed. A detection system was constructed using loop-mediated isothermal amplification and optimized. The established method was analyzed for its specificity and limit of detection, and compared with the conventional methods （based on isolation and subsequent culture）. Results： The detection method and kit developed in this study was specific to Listeria monocytogenes detection in meat product and presented negative results for other related species. The limit of detection was 1.81 × 10^3 CFU/mL, and the sensitivity was consistent with that of the conventional methods. Other advantages such as simple and rapid detection and few needs for instruments were also achieved, thereby deserving to be popularized.关键词
恒温核酸扩增/环介导恒温扩增（LAMP）/肉制品/微生物/食品安全Key words
loop-mediated isothermal amplification （LAMP）/meat product/microbiological/food safety分类
轻工纺织引用本文复制引用
陈洵,冯雪梅,杜正平,谭慧媚,田文武,廖秀志,曹以诚..环介导恒温扩增法检测肉制品中致病微生物[J].肉类研究,2011,(11):29-33,5.
