肉类研究Issue(12)：22-24,3.
一种提取肉类总DNA的方法——改良CTAB法
An Optimized CTAB Method for Total RNA Extraction from Meat
摘要
Abstract
An optimized CTAB method for total RNA extraction was presented for use in accurately quantifying components of animal origin and fighting against adulterated food in the market as a sample preparation method. The use of the CTAB method to extract total RNA from pork was investigated with respect to sample amount, dissociation time, precipitation time and RNase treatment. As a result, a fast and efficient method for total RNA extraction was achieved by selecting sample amount below 0.05 g, 0.5 h dissociation, 0.5h precipitation and non-RNase treatment. The method showed a good linear relationship （R2 = 0.9972） and stability （SD = 0.18）.关键词
DNA提取/改良CTAB法/肉类Key words
DNA extraction/optimized CTAB method/meat分类
轻工纺织引用本文复制引用
周彤,李家鹏,田寒友,杨君娜,乔晓玲..一种提取肉类总DNA的方法——改良CTAB法[J].肉类研究,2011,(12):22-24,3.基金项目
国家“863”计划项目 （）
北京市科技计划项目 （）
