肉类研究Issue(12)：25-28,4.
适于肉种掺杂比例实时荧光PCR测定的样品前处理方法
An Optimized Sample Pretreatment Method for Quantitative Detection of Meat Adulteration by PCR
摘要
Abstract
In this study, an L9（34） orthogonal array design was employed to optimize homogenization conditions for use in the sample pretreatment for quantitative detection of meat adulteration by PCR. Meanwhile, the effect of sample homogeneity on the accuracy of PCR detection was examined using three different sampling amounts： 30, 10 g and 3 g of rump pork. The optimal homogenization conditions were found to be： 1：4 （m/V） meat/water ratio, 12000 r/min homogenizer rotary speed and 8 min homogenization time. Under these conditions, a good homogeneity was obtained for pretreated samples, and the results of determination of sample mass, DNA concentration and real-time PCR Ct also showed a good homogeneity and no significant difference. Further, the optimized homogenization conditions were used to detect pork at the three sampling levels above, with the result that sampling amount had no effect on sample homogeneity.关键词
肉种掺杂比例/实时荧光PCR实验/前处理方法/样品均匀性Key words
meat adulteration ratio/RT-PCR array/pretreatment method/sample homogeneity分类
生物科学引用本文复制引用
杨君娜,李家鹏,周彤,田寒友,乔晓玲..适于肉种掺杂比例实时荧光PCR测定的样品前处理方法[J].肉类研究,2011,(12):25-28,4.基金项目
北京市科技计划项目 （）
国家“863”计划项目 （）
