肉类研究Issue(12)：29-32,4.
酶联免疫法检测猪肉中呋喃唑酮代谢物残留
Determination of Furazolidone Metabolite Residue in Pork by Enzyme-linked Immunosorbent Assay
闵成军 1罗晓琴 2汪善良 2崔廷婷2
作者信息
- 1. 雨润集团,江苏南京210041
- 2. 北京勤邦生物技术有限公司,北京102206
- 折叠
摘要
Abstract
An enzyme-linked immunosorbent assay （ELISA） was proposed to determine residual furazolidone metabolite in pork using a commercially available ELISA kit. The results showed that the sensitivity and detection limit of the assay were 0.025-- 2.025 μg/L and 0.1 μg/kg, respectively. The recoveries of standard additions of furazolidone metabolite averaged 78.2% -- 100.3% across three levels （n = 3）, and the coefficients of variation were 4.7%--10.3%. The cross-reactivity towards furazolidone was 16.3%; as for other drags investigated, the cross-reactivity was below 1%. Analytical results obtained using the assay were in substantial accordance with those from liquid chromatographic tandem mass spectrometry （LC-MS/MS） determination. The assay was sensitive, accurate, repeatable, specific and suitable for the determination of furazolidone metabolite residue in pork.关键词
呋喃唑酮代谢物/酶联免疫法/猪肉Key words
fnrazolidone metabolite （AOZ）/enzyme-linked immunosorbent assay （ELISA）/pork分类
轻工纺织引用本文复制引用
闵成军,罗晓琴,汪善良,崔廷婷..酶联免疫法检测猪肉中呋喃唑酮代谢物残留[J].肉类研究,2011,(12):29-32,4.
