乳业科学与技术2011，Vol.34Issue(5)：201-206,6.
响应面法优化德氏乳杆菌保加利亚亚种NCFB2772发酵生产胞外多糖的培养条件优化
Employing Response Surface Methodology to Optimize Culture Conditions for Extracellular Polysaccharide Production by Lactobacillus delbrueckii snbsp.bulgaricus NCFB2772
摘要
Abstract
Extracellular polysaccharide （EPS） and strategies for improving ESP yield and functionality are always a hot topic that has received much attention in the academic world. In this study, response surface methodology was employed to optimize the culture conditions for EPS production by Lactobacillus delbrueckii subsp.bulgaricus NCFB2772 in MRS medium. Based on one-factor-at-a-time experiments, a 6-factor, 2-level Plackett-Burman factional design was performed to identify initial medium pH （C1）, inoculum size （C2） and culture temperature （C3） as main factors that affect EPS production （Y）. Further, the selected cuRure conditions were optimized for achieving maximum EPS production using response surface methodology based on a 3- factor, 3-level central composite design. It was found that initial medium pH 6.5 and 4.5% inoculum size for fermentation at 42 ℃ provided maximum EPS production （907.8 mg/L）. A regression model was built as follows： Y= - 287.08 ＋ 39.83C1 ＋ 11.44C2 ＋ 18.54C3. This study has the potential to provide references for improved industrial EPS production from Lactobacillus delbruecldi subsp.bulgaricus NCFB2772.关键词
响应面法/德氏乳杆菌保加利亚亚种NCFB/胞外多糖（EPS）/培养条件Key words
response surface methodology/LactobaciUus delbrueckii subsp.bulgaricus NCFB2772/extracellular polysaccharide （EPS）/culture conditions分类
生物科学引用本文复制引用
汪清美,陈庆森,阎亚丽..响应面法优化德氏乳杆菌保加利亚亚种NCFB2772发酵生产胞外多糖的培养条件优化[J].乳业科学与技术,2011,34(5):201-206,6.基金项目
国家自然科学基金面上基金项目 （）
