食品工业科技Issue(9)：203-205,209,4.
冷冻干燥对乳酸菌代谢活力的影响
Effect of freeze drying on the metabolic viability of lactic acid bacteria
摘要
Abstract
The survival rates of Lactobacillus plantarum ST-Ⅲ and Lactobacillus bulgaricus in the presence of cryoprotectants such as 15%sucrose,10%trehalose,15%sucrose ＋2% sodium glutamate and 10%reconstituted skim milk（RSM）were estimated.In addition,we determined the activities of hexokinase（HK）,pyruvate kinase（PK）,lactate dehydrogenase（LDH）,extracellular proteins was higher and intercellular Ca2＋ immediately following the freeze-drying.The results showed that the differences in HK and PK activities with and without the cryoprotectants during freeze-drying were not significant,but activities of LDH were significantly different prior to and after freeze-drying.Meanwhile,the results showed extracellular proteins was higher and intercellular Ca2＋ was lower in absence of cryoprotectants than in the presence of cryoprotectants.These results suggested that freeze-dying inactivated key enzymes（LDH）and damaged membrane structure and function.These led to decrease in viability of lactic acid bacteria during freeze-drying.关键词
冷冻干燥/乳酸菌/保护剂/乳酸脱氢酶/膜损伤Key words
freeze-drying/lactic acid bacteria/cryoprotectants/lactate dehydrogenase/damage of membrane分类
轻工纺织引用本文复制引用
李宝坤,田丰伟,刘小鸣,赵建新,张灏,宋元达,陈卫..冷冻干燥对乳酸菌代谢活力的影响[J].食品工业科技,2011,(9):203-205,209,4.基金项目
国家自然基金 （）
“十一五”国家科技支撑计划重点项目 （）
