食品工业科技Issue(9)：223-226,4.
Plackett-Burman设计和响应面法优化火红密孔菌发酵产漆酶培养基
Optimization of culture medium for laccase production from Pycnoporus sanguineus（Fr.）Murr by Plackett-Burman design and response surface methodology
摘要
Abstract
Objective：Drawing assistance from MINITAB 15.0 software,Plackett-Burman experimental design and response surface methodology were applied to optimizing culture medium for laccase production from Pycnoporus sanguineus（Fr.）Murr.Method：The important factors influencing laccase production,which identified by initial experimental design of Plackett-Burman were wheat bran,glucose and peptone.The path of steepest ascent was undertaken to approach the optimal region of the three significant factors.Box-Behnken design and response surface analysis were adopted to further investigate the mutual interaction between the variables and obtain optimal values that bring maximum laccase activity.Results：The results showed that wheat bran,glucose and peptone all had significant influence on the activity of laccase.The optimal conditions for the maximal activity discriminated from the regression equation were：wheat bran 2.5%,glucose 2.0%,peptone 1.2%,natural pH.The maximum value was 330.66U/mL.Compared with the laccase activity（238U/mL）cultured in fermentation medium it increased 38.9% under the optimal conditions.Conclusion：Culture medium for Laccase production from Pycnoporus sanguineus（Fr.）Murr was optimized for the first time using statistics and the mathematical model for laccase production was established.关键词
漆酶/响应面法/Plackett-Burman/火红密孔菌Key words
laccase/response surface methodology/Plackett-Burman/Pycnoporus Sanguineus（Fr.）分类
轻工纺织引用本文复制引用
张田田,沈明浩..Plackett-Burman设计和响应面法优化火红密孔菌发酵产漆酶培养基[J].食品工业科技,2011,(9):223-226,4.基金项目
吉林省科技厅课题 （）
