食品工业科技Issue(9)：407-410,4.
化学发光酶免疫法检测玉米中脱氧雪腐镰刀菌烯醇
Chemiluminescence enzyme immunoassay for the determination of deoxynivalenol in corn
摘要
Abstract
A sensitive indirect competitive chemiluminescence＇s enzyme immunoassay（CLEIA）for deoxynivalenol in corn was developed. The optimized CLEIA gave a limit detection of 10. 7ng/mL and a detection range of 12. 9～163. 7ng/mL,with an IC50 of 46. 01ng/mL and it was about 4. 01 times more sensitive than that of colorimetric ELISA. It had been validated on cereals samples in terms of precision（intra-and interassay coefficient variations of less than 8. 1% and 12. 8%,respectively）and accuracy（mean recovery 91. 2%～99. 5%）. These assay performances indicated that CLEIA was capable of being applied for the specific detection and routine monitoring of DON in corn samples.关键词
脱氧雪腐镰刀菌烯醇（DON）/间接竞争化学发光酶免疫法（CLEIA）/检测Key words
deoxynivanol/CLEIA/detection分类
轻工纺织引用本文复制引用
楚金申,许杨,何庆华,卢江..化学发光酶免疫法检测玉米中脱氧雪腐镰刀菌烯醇[J].食品工业科技,2011,(9):407-410,4.基金项目
“国家高技术研究发展计划”项目资助 （）
