食品工业科技Issue(9)：420-422,3.
啤酒中嘌呤的纯化及反相离子对色谱法测定的研究
Study on purification of purines in beer and determination of purines compounds by reversed-phase ion pair chromatography
摘要
Abstract
Four purines（Gua,Ade,Xan and Hyp）were separated and determined by reversed-phase ion pair chromatography.The optimized chromatography conditions were as following：254nm by UV detector with 0.02mol/L of KH2PO4 buffer solution added 1mmol/L 1-Hexanesulfonic acid sodium（pH=3.6 regulated by H3PO4 and methanol concentration=3%（v/v）regulated by quaternary pump）as mobile phase,and flow rate at 1.0mL/min.The average recoveries of four purines were 95.97%～101.74%,with the accuracy of the method as RSD of all four purines were 1.21%～2.04%.This method provided more feasibility than normal reversed-phase chromatography as to the purines including ion and non-ionic compound.The chromatography of beer sample was without interfering peak almost after beer sample purified by precipitation method,because of that determine the chemical composition and amount became simple and accurate.关键词
啤酒/嘌呤/反相离子对色谱/纯化Key words
beer/purine/reversed-phase ion pair chromatography/purification分类
轻工纺织引用本文复制引用
何炘,陈静波,刘绘景,刘华..啤酒中嘌呤的纯化及反相离子对色谱法测定的研究[J].食品工业科技,2011,(9):420-422,3.基金项目
浙江省质监系统科研计划项目 （）
