食品工业科技Issue(10)：249-252,4.
嗜热真菌发酵产木聚糖酶培养基的优化及部分酶学性质的研究
Optimization of fermentation medium for thermophilic fungal xylanase and study on some enzymatic properties
张新峰 1王伟平 2汪蓉 1王琛琛 2阳飞 3张华山1
作者信息
- 1. 发酵工程省部共建教育部重点实验室
- 2. 湖北工业大学,湖北武汉430068
- 3. 湖北大学知行学院,湖北武汉430011
- 折叠
摘要
Abstract
Fermentation medium of thermophilic fungal for xylanase production was optimized by response surface method and the basic properties of the enzyme solution were studied. First,eight kinds of factors of the initial medium was adopted by the Plackett-Burman design,and selected three kinds of important factors, namely,corn cob,urea and KH2PO4,and then the path of steepest ascent was adopted for the central composite experiment to determine the maximum response interval. Finally response surface optimization results was obtained ,corncob 4.570% （w/v）,urea 1.797% （w/v）, KH2PO4 0.497% （w/v）,therefore,enzyme activity was increased by 18%. The enzyme pecificity was better,corncob being regard as substrate,the optimal enzyme reaction temperature was 65℃ and the temperature was more stable,the optimum pH 6.8 and pH stability was better.关键词
嗜热真菌/木聚糖酶/优化/酶学性质Key words
Thermophilic fungi/xylanase/optimization/enzymatic properties分类
轻工纺织引用本文复制引用
张新峰,王伟平,汪蓉,王琛琛,阳飞,张华山..嗜热真菌发酵产木聚糖酶培养基的优化及部分酶学性质的研究[J].食品工业科技,2011,(10):249-252,4.
