食品工业科技Issue(11)：194-197,4.
朱黄青霉α-1,6-葡聚糖酶在毕赤酵母中的分泌表达
Expression of α-1,6-dextranase from Penicillium minioluteum in P.pastoris
摘要
Abstract
α-1,6-dextranase,which can hydrolyze dextran specifically by cutting off the α-1,6-glycosidic bond to release shorter saccharides,was widely used in many fields such as sugar industry and beer industry.The gene of α-1,6-dextranase（dex）was amplified through PCR by using Penicillium minioluteum C12114 genomic DNA as template.The amplified gene was cloned into vector pPIC9K and the recombinant plasmid pPIC9K-dex was linearzed with Sac I,then transformed into P.pastoris GS115 by electroporation.The positive transformant was induced to express the enzyme with 1.5% methanol for 7 days under the 30℃,and the activity of the enzyme could reach 88.35U/mL.关键词
α-1/6-葡聚糖酶/朱黄青霉/毕赤酵母/诱导Key words
α-1/6-dextranase/Penicillium minioluteum/P.pastoris/induction分类
轻工纺织引用本文复制引用
庄灵习,段然,陈龙军,凌雪萍,卢英华..朱黄青霉α-1,6-葡聚糖酶在毕赤酵母中的分泌表达[J].食品工业科技,2011,(11):194-197,4.基金项目
广东省科技计划项目 （）
