食品工业科技Issue(11)：426-430,5.
免疫亲和净化-超高效液相色谱串联质谱法同时测定猪肉和鸡蛋中的12种磺胺类药物
Immunoaffinity chromatography purification and ultra high performance liquid chromatography tandem mass spectrometry method for simultaneous determination of twelve kinds of sulfonamides in pork and egg
摘要
Abstract
A simultaneous determination method for twelve kinds of sulfonamides in pork and egg was developed using immunoaffinity chromatography（IAC）and ultra-high performance liquid chromatography-tendem mass spectrometry（UPLC-MS/MS）.Samples were ultrasonicated extracted,and then the extracts were pooled and cleaned up with immunoaffinity column,followed by blowing to dryness under nitrogen gas stream,reconstitution with methanol/water mixture（1∶ 9,v/v）before the analysis on UPLC-MS/MS.The MS conditions used were positive（ESI＋）electrospray ionization and MRM models.The results indicated as follows：All the studied sulfonamides had good linear relationship with peak area over the range from 10μg/kg to 200μg/kg,the detection limits of instrument for them ranged from 0.4μg/L to 2.0μg/L and the detection limits of method for them ranged from 1.0μg/kg to 5.0μg/kg;and the average recoveries rates in the above two matrices spike at three levels were between 67% and 100%,with relative standard deviations（RSDs）varying from 0.9% to 10.0%.This analytical method meets the requirements for trace assays.关键词
超高效液相色谱-串联质谱法/免疫亲和/猪肉/鸡蛋/磺胺类药物Key words
ultra high performance liquid chromatography tandem mass spectrometry/immunoaffinity/pork/egg/sulfonamides分类
轻工纺织引用本文复制引用
赵笑天,潘亚利,傅英文,宋欢,林勤保..免疫亲和净化-超高效液相色谱串联质谱法同时测定猪肉和鸡蛋中的12种磺胺类药物[J].食品工业科技,2011,(11):426-430,5.基金项目
山西省科技攻关计划 （）
