食品工业科技Issue(11)：443-446,450,5.
响应面分析法优化抑制-褪色光度法测定维生素C的条件
Study on optimization of conditions of determination of vitamin C by inhibition discoloring spectrophotometry using response surface methodolgy
摘要
Abstract
Base on the inhibition effect of Vitamin C on discoloring reaction between Rhodamin B and K2S2O8 by Ag＋ catalysis,response surface methodology（RSM）was emplyed to optimize the condition（temperature,time,dosage of RB,dosage of HAc）.The result of RSM showed that the optimum condition was as fllows：reaction temperature 20.0℃,reaction time 11.82min,dosage of RB 6.99mL and dosage of HAc 2.81mL.Under these condition,the content of Vitamin C from Tragopogon porrifolius L.is 0.08mg/g.The results showed that recovery of standard addition was 100.97%,and the RSD（n=5）was 0.25%.关键词
响应面法/抑制褪色/VC/条件实验Key words
RSM/discoloring reaction/Vitamin C/condition test分类
轻工纺织引用本文复制引用
王巧英,吴冬青,安红钢,宋海,任雪峰..响应面分析法优化抑制-褪色光度法测定维生素C的条件[J].食品工业科技,2011,(11):443-446,450,5.基金项目
甘肃省教育厅（1009-09）科研资助项目 （）
