食品工业科技Issue(11)：447-450,4.
固相萃取-分光光度法快速检测腐竹中的碱性橙
Rapid determination of basic orange in dried beancurd sticks by solid phase extraction-spectrophotometry
摘要
Abstract
Objective：A novel method based on solid phase extraction followed by Spectrophotometry was developed for the analysis of prohibited colorant basic orange in dried beancurd sticks.Method：Basic orange in dried beancurd sticks was extracted with SIPORE SPE-C18SPE column,purified by 20% methanol aqueous solution（V/V）,eluted by 80% methanol aqueous solution（V/V）and detected by Spectrophotometry with mensurated at the maximum absorption wavelength 446nm.Results：The linear correlation coefficient was 0.9955 over the range of 2.0 ~ 10.0μg/mL.The recoveries were 102.24% ~ 84.15% for spiked standards at 2.0 ~ 8.0μg/mL level.The average recovery of repeated test was 98.45% with relative standard deviation（RSD）of 1.87 %（n=5）.Conclusions：This method with good linearity,high precision and rapidity was suitable for rapid determination of basic orange in dried beancurd sticks.关键词
固相萃取/分光光度法/碱性橙/腐竹Key words
solid phase extraction/spectrophotometry/basic orange/dried beancurd sticks分类
轻工纺织引用本文复制引用
李小燕,仝海娟,石展望,罗少芹,秦玉凤,李梅..固相萃取-分光光度法快速检测腐竹中的碱性橙[J].食品工业科技,2011,(11):447-450,4.基金项目
广西自然科学基金项目 （）
广西教育厅科研项目 （）
