食品工业科技Issue(12)：261-264,396,5.
黑曲霉ATCC16404固体发酵豆皮产生木聚糖酶的研究
Xylanase production by Aspergillus niger ATCC16404 in solid-state fermentation of soybean hull
摘要
Abstract
Components of incubation media and followed by the best conditions for enzyme production were optimized in solid-state fermentation of soybean hull by Aspergillus niger ATCC16404 to produce xylanase,whose activity was determined by DNS. Results of single factor experiments showed that the optimum components of incubation media contain ammonium sulfate and magnesium sulfate used as nitrogen source and mineral element,with 2% addition based on the weight of 25～50 mesh dry soybean hull,and without additional carbon source and surfactant. Solid-to-liquid ratios,initial pH,incubation time,inoculum size were selected to optimize the condition of enzyme production. Orthogonal experiment showed the highest xylanase activity was 366. 29U/g at the optimum fermentation conditions of solid-to-liquid ratio 1∶ 1. 00,initial pH 5. 5,3d incubation,0. 3mL /10 g spore suspension of 1. 0×107/mL concentration.关键词
黑曲霉/豆皮/木聚糖酶/固体发酵Key words
Aspergillus niger/soybean hull/xylanase/solid-state fermentation分类
轻工纺织引用本文复制引用
李爱军,蒋文明,欧仕益,孙远明..黑曲霉ATCC16404固体发酵豆皮产生木聚糖酶的研究[J].食品工业科技,2011,(12):261-264,396,5.基金项目
广东高校科技成果产业化重大项目 （）
