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芦根多糖的分离纯化和体外抗肿瘤研究

晁若瑜 杨靖亚 蔡晓晔 陆晓帆 钱媛华

食品工业科技Issue(12):284-286,3.
食品工业科技Issue(12):284-286,3.

芦根多糖的分离纯化和体外抗肿瘤研究

Extraction,purification and anti-tumor activity of Reed Rhizome polysaccharide

晁若瑜 1杨靖亚 1蔡晓晔 2陆晓帆 1钱媛华1

作者信息

  • 1. 上海海洋大学,上海201306
  • 2. 上海海洋大学海洋科学研究院,上海201306
  • 折叠

摘要

Abstract

The polysaccharide was extracted from Reed Rhizome,the technology of its isolation,purification and anti- tumor activity were also studied. The polysaccharide was obtained through hot water extraction,then protein was removed by means of Sevag methods and purified by Sephadex chromatography. And the vitro anti-tumor activities were analyzed with cytotoxicity. The results showed that the saccharide content of the sample was 0. 872%. Three kinds of polysaccharide(R-PolyⅠ,R-PolyⅡ,R-PolyⅢ)were obtained through the isolation and purification procedure,with their molecular weight estimated as 79781,29073,10605u respectively. Besides,the three polysaccharides displayed a noticeable inhibition effect in Hela and B16 cells. It laid the foundation for the wide application of the R-Poly in such fields as food industry and health care.

关键词

芦根多糖/提取/纯化/抗肿瘤

Key words

Reed Rhizome polysaccharide/extraction/purification/anti-tumor

分类

轻工纺织

引用本文复制引用

晁若瑜,杨靖亚,蔡晓晔,陆晓帆,钱媛华..芦根多糖的分离纯化和体外抗肿瘤研究[J].食品工业科技,2011,(12):284-286,3.

基金项目

上海市教育委员会科研创新项目 ()

上海市教育委员会重点学科建设项目 ()

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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