食品工业科技Issue(12)：415-417,423,4.
肠致病性大肠杆菌O128∶K67（B12）的脉冲场凝胶电泳分型方法研究
Molecular typing of enteropathogenic Escherichia coil O128∶ K67（B12） by pulsed field gel electrophoresis
摘要
Abstract
Objective：To investigate the genotypic diversity in 7 EPEC O128∶ K67（B12）isolates from raw milk samples and cow dung samples collected in 9 cattle farms in Tianjin city and 9 cattle farms in Baoding city. Methods：PFGE was used to study genotypic variability of the 7 EPEC O128∶ K67（B12）isolates obtained. Results：For these isolates,when XbaI was used to chromosomal DNA digestion followed by PFGE analysis,17～20 genomic fragments were separated in each PFGE profile obtained. And the 7 strains were divided into 3 clusters by the dendrogram analysis of all the PFGE profiles obtained. Conclusions：7 EPEC O128∶ K67（B12）strains were divided into 3 clusters,and strains of each cluster had no close relationship with those of another. There was no evidence that the strains of any cluster were prevalent. In addition,PFGE as a prospective method for sub typing pathogens and analyzing the homology of the pathogens in raw milk was shown to be highly discriminating,reliable,feasible,and advanced.关键词
肠致病性大肠杆菌O128∶K67（B12）/脉冲场凝胶电泳/分型Key words
enteropathogenic Escherichia coil O128∶ K67（B12）/pulsed field gel electrophoresis/molecular typing分类
轻工纺织引用本文复制引用
张佰荣,孟祥晨..肠致病性大肠杆菌O128∶K67（B12）的脉冲场凝胶电泳分型方法研究[J].食品工业科技,2011,(12):415-417,423,4.基金项目
“十一五”国家科技支撑计划（2006BAD04A08）资助项目 （）
