食品工业科技Issue(12)：462-464,467,4.
荧光衍生化法测定不同季节桑叶中1-脱氧野尻霉素的含量
Determination of 1-deoxynojirimycin in Morus alba L.leaves of different seasons by fluorometric derivatization
摘要
Abstract
Objective：To develop a high performance liquid chromatographic method for the determination of 1-deoxynojirimycin（DNJ）in Morus alba L. leaves and contrast the content of 1-DNJ in different seasons. Methods：The separation was performed with a Kromasil C18 column（4. 6mm×250mm,5μm）at 30℃ and the mobile phase consisted of acetonitrile-0. 1%aqueous acetic acid（50∶ 50）,at the rate of 1. 0mL/min. The 1-DNJ marked with 9-fluorenylmethyl chloroformate（FMOC-Cl）was detected at excitation wavelength of 254nm and emission wavelength of 322nm. Results：The calibration curve was linear over the concentration range from 10mg/L to 35mg/L（r=0. 99959）,the limit of detection was 0. 80mg/L（S/N=3）and the average recovery was 95. 38%（RSD=2. 28%）. The results showed that the content of 1-DNJ was significantly different in different seasons. Conclusion：This method was simple,rapid,and accurate and was suitable for the determination of 1-DNJ in Morus alba L. leaves.关键词
桑叶/1-脱氧野尻霉素/高效液相色谱法/荧光衍生化Key words
Morus alba L. leaves/1-deoxynojirimycin/high performance liquid chromatography/fluorometric derivatization分类
轻工纺织引用本文复制引用
李继文,郭晓燕,王腾,李翠清..荧光衍生化法测定不同季节桑叶中1-脱氧野尻霉素的含量[J].食品工业科技,2011,(12):462-464,467,4.基金项目
教育部科学技术研究重点项目 （）
北京市教育委员会科技发展计划面上项目 （）
