食品工业科技Issue(12)：473-475,3.
高效液相色谱串联质谱法测定鲢鱼中的微囊藻毒素
Determination of microcystins in fish by high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
Microcystins（MC-RR,MC-LR and MC-YR）were determined by high performance liquid chromatography（HPLC）combined with electrospray ionization and tandem mass spectrometry（ESI-MS/MS）. Microcystins were extracted with methanol from fish,and purified by solid-phase extraction on the C18 cartridge column. Chromatographic separation was performed on C18 column with methanol and 0. 1% of formic acid solution as mobile phase,gradient elution with using C18 column separated the microcystins from fish samples,by electrospray mass spectrometry choice model for testing. The working curves for MC-RR,MC-LR,MC-YR were good linearity,and the method detection limit were 0. 2,0. 4,0. 4μg·L-1,and quantization limits were 0. 8,0. 9,0. 9μg·L-1. The recovery rates for microcystins in fish samples were 87. 1%～101. 2%,and 10 parallel determination of the relative standard deviation was less than 8%. This method was rapid,simple and accurate,high sensitivity for measurements of MCs. It could adapt to quick analysis the large samples.关键词
微囊藻毒素/效液相色谱串联质谱法（HPLC-MS/Ms）/检测Key words
microcystins/HPLC-MS/MS/determination分类
轻工纺织引用本文复制引用
陈长毅,陈玉波,于文芹..高效液相色谱串联质谱法测定鲢鱼中的微囊藻毒素[J].食品工业科技,2011,(12):473-475,3.基金项目
国家科技部星火计划项目 （）
