食品工业科技Issue(12)：486-488,3.
褐藻寡糖分子量测定方法的研究
Study on the determination method of molecular weight of alginate oligosaccharide
摘要
Abstract
A rapid and accurate determination method of molecular weight of alginate oligosaccharide was studied. Alginate oligosaccharide A and B were degradated by oxidation and high pressure,and their molecular weight were determined respectively by ubbelohde viscometer and high performance liquid chromatography（HPLC）. It was showed that,molecular weights of A and B were just 7300 and 5050,by ubbelohde viscometer,which were 10666 and 7935 however by HPLC. The molecular weights determined by two different methods coincided with each other but varied greatly. It was because that,the molecular weight determined by ubbelohde viscometer was calculated in empirical equation,not the absolute value;however,by HPLC,the linear regression equation was established between the log number of molecular weight（lgMw）and the retention time（t）：lgMw=-2. 0338t＋25. 834,R2=0. 9926,which returned to a good relation. In summary,HPLC was precise,efficiency,smart and simple,which was more suitable for the determination,and provided a theoretical and empirical basis for the molecular weight determination of alginate oligosaccharide.关键词
褐藻寡糖/分子量/乌式黏度法/高效液相色谱法Key words
alginate oligosaccharide/molecular weight/ubbelohde viscometer/high performance liquid chromatography分类
轻工纺织引用本文复制引用
赵珊,许加超,付晓婷,高昕..褐藻寡糖分子量测定方法的研究[J].食品工业科技,2011,(12):486-488,3.基金项目
海洋食品精深加工技术研究与产业化示范 （）
