基于抗体微阵列的食源性病原体综合检测平台OA北大核心CSCDCSTPCD
Comprehensive platform for detecting foodborne diseases pathogens based on antibody microarrays
针对超低浓度食源性病原体样本检测的需要,将表面沉积铁氰酸镍薄膜的微叉指电极与生物活化的微珠相结合,设计并实现了通过测定电化学阻抗变化的抗体微阵列食源性病原体综合检测平台样件。提出了电化学阻抗等效电路,并通过电路模型分析表明,生物活化的微珠、微叉指电极之间的电容、溶液的电阻均对电化学阻抗传感单元的输出特性有显著影响。以不同浓度的病原体为样本,完成了Escherichia coli O157∶H7为目标病原体的实验研究。实验结果表明,该微抗体阵列的检…查看全部>>
With the aim to solve the difficulty in detection of ultra-low level foodborne disease pathogens on the spot,a comprehensive antibody microarray platform for rapid detection of multiple foodborne disease pathogens had been designed and realized. By combination of interdigitated microelectrodes(IDMs)deposited with thin nickel hexacyanoferrate film and bioactivated microbeads,the electrochemical impedance(ECI)cell had been rendered with a good sensitivity. An …查看全部>>
林荣生
厦门华厦职业学院食品药品安全检测中心,福建厦门361024
轻工纺织
食源性病原体抗体微阵列超低浓度电化学阻抗生物活化微珠
foodborne pathogensantibody microarrayultra-low levelelectro-chemical impedancebioactivated microbeads
《食品工业科技》 2011 (12)
489-493,5
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