食品工业科技Issue(12)：494-496,3.
淮山药薯蓣皂苷测定方法的研究
Study on the determination method of dioscin in Dioscorea opposita Thunb
韩秋敏 1李登超 2黄亚东 1韩正亮2
作者信息
- 1. 江苏食品职业技术学院生物与化学工程学院,江苏淮安223003
- 2. 淮阴师范学院生命科学学院,江苏淮安223300
- 折叠
摘要
Abstract
Commonly used ultraviolet-visible spectrophotometry methods,vanillin-perchloric acid method,vanillin-sulfuric acid and methanol-sulfuric acid method,for determination content of dioscin were comparatively studied. The results showed that compounds formed under different treatments were very different at the maximum absorption wavelength and their stabilities. The methanol-sulfuric acid method was good,and the maximum absorption wavelength was 332nm,with good stabilities within 60min. The calibration curve was linear in the range of 2. 4～24μg/mL. Average content of dioscin in Dioscorea opposita Thunb was 0. 535mg/g with RSD 1. 06% by the methanol-sulfuric acid method. This method was simple,accurate,sensitive and stable,which could be used for the quantitative determination of total dioscin in Dioscorea opposita Thunb.关键词
淮山药/薯蓣皂苷/比色法Key words
Dioscorea opposita Thunb/dioscin/colorimetry分类
轻工纺织引用本文复制引用
韩秋敏,李登超,黄亚东,韩正亮..淮山药薯蓣皂苷测定方法的研究[J].食品工业科技,2011,(12):494-496,3.
