食品工业科技Issue(1)：200-203,4.
一次过柱法快速分离纯化驴心肌中高铁肌红蛋白
Extraction and purification of metmyoglobin from donkey（Equus asinus） heart muscle by single-step chromatography
摘要
Abstract
Myoglobin and its existing states are responsible for the color of meat and meat products,of which metmyoglobin is an important one.Myoglobin was extracted from donkey（Equus asinus） heart muscle by ammonium sulfate fractionation（70%～100%）.After oxidized by potassium ferricyanide and dialyzed,the sample solution was further purified by CM-Sephadex Fast Flow ion exchange column chromatography.The results of SDS-PAGE electrophoresis and absorption spectra analysis showed that metmyoglobin in electrophoresis pure was obtained by single column chromatography.This provided a fast and efficient way for extraction and purification of metmyoglobin.关键词
驴心肌/高铁肌红蛋白/纯化/电泳纯Key words
donkey heart muscle/metmyoglobin/purification/electrophoresis pure分类
轻工纺织引用本文复制引用
李沛军,孔保华,郑冬梅,陈倩..一次过柱法快速分离纯化驴心肌中高铁肌红蛋白[J].食品工业科技,2012,(1):200-203,4.基金项目
国家公益性行业（农业）科研专项经费项目 （）
东北农业大学创新专项基金 （）
