食品工业科技Issue(1)：238-240,3.
高纯度低聚半乳糖单一组分的制备研究
Study on preparation of high purity of galacto-oligosaccharides single components
摘要
Abstract
Bio-Gel P-2 gel filtration chromatographic column was used for the purification of Galacto-oligosaccharides（GOS） mixture,by which the effect of column temperature,elution flow-rate and sample volume on the separation were studied.The results showed that the optimum separation could be obtained at 50℃,15～20mL/h and sample volume 3mL with the column of 1.6cm×120cm and elution solution of degassed distilled water.Products of tri-,tetra-and pentasaccahride with the purity of more than 85% were achieved under this condition.Followed the second column chromatography for the concentrate of above single products,more than 98% purity and the yield of 95.5%,74.4% and 96.7% of tri-,tetra-and pentasaccharide,respectively,were obtained,which could meet the requirements of the standardized sample.关键词
聚丙烯酰胺凝胶/Bio-Gel/P-2/低聚半乳糖/柱层析Key words
polyacrylamide gel/Bio-Gel P-2/galacto-oligosaccharides/column chromatograph分类
轻工纺织引用本文复制引用
韩晓丽,苏芳云,冯咏梅,常秀莲,张瑞伦,刘庚鑫..高纯度低聚半乳糖单一组分的制备研究[J].食品工业科技,2012,(1):238-240,3.基金项目
山东省科技攻关项目 （）
