食品工业科技Issue(2)：52-54,84,4.
生物传感器在脂肪酶酯化反应过程中的应用
Application of biosensor in the process of lipase esterification
摘要
Abstract
An electrochemical biosensor was designed and developed which could transform the electron metastasis process produced by lipase conjugating the substrate to current output in order to accurately reflect the diversification of regular pattern of lipase in the process of catalytic esterification.Novozym 435 and Lipozyme TL IM were used as biological components respectively, electric current in the process of esterification of CLA and glycerin was collected, liquid chromatography was used to determine the content of the substrate of the reaction which could verify the degree of agreement between the process of current changing and esterification.The relative error was 4.7% by making experiments.It proved that we could judge the extent of the esterification conjugated by lipase by detecting the current changing in the process of the reaction which provided a basic premise for realizing the continuous detection on-line by computer.关键词
脂肪酶/生物传感器/电流/相对误差/反应程度Key words
lipase/biosensor/electric current/relative error/reaction content分类
轻工纺织引用本文复制引用
刘滨城,齐颖,李越,任运宏,于殿宇..生物传感器在脂肪酶酯化反应过程中的应用[J].食品工业科技,2012,(2):52-54,84,4.基金项目
国家863高技术研究发展计划（2010AA101503）. （）
