食品工业科技Issue(2)：199-202,4.
应用DPO技术检测食源性致病菌的多重PCR的研究
Multiplex-PCR for detection of foodborne pathogens with a dual priming oligonucleotide（DPO） system
摘要
Abstract
DPO is one of the new specific primers. In this study, four sets of dual priming oligonucleotide（DPO） primers were selected according to Listeria monocytogenes prfA gene, E.coli O157 ： H7 rfbE gene, Vibrio parahaemolyticus tl gene, Shigella ipaH gene, respectively, and applied in the detection of the four previously mentioned foodborne pathogens with multiplex polymerase chain reaction（mPCR）.The results showed that DPQ- mPCR exhibited high PCR specificity even under less than optimal PCR condition compared with conventional mPCR.Meanwhile,this method is easy to operate.关键词
DPO引物/多重PCR/食源性致病菌Key words
DPO primers/mPCR/foodborne pathogens分类
生物科学引用本文复制引用
卢富山,王云龙,景建洲..应用DPO技术检测食源性致病菌的多重PCR的研究[J].食品工业科技,2012,(2):199-202,4.基金项目
河南省杰出青年基金项目（74100510007）. （）
