食品工业科技2012，Vol.33Issue(3)：99-101,105,4.
大豆抗癌肽的分离纯化及分子量分布
Purification and distribution of molecular weight of soybean anti-cancer peptides
摘要
Abstract
To determine the molecular weight of soybean peptides with anti-cancer activity,the extraction of soybean anti-cancer peptides was ultrafiltrated.MTT（thiazole blue four） assay was used to detect the anti-cancer activity of different components obtained by ultrafiltration.Components of soybean anti-cancer peptides with molecular weight ranging from 5000 to 10000u had the highest anti-cancer activity,and the IC50 of soy anti-cancer peptides was 0.47mg/mL.Gel Filtration Chromatography（Sephadex G-75） was applied to further separate and collect all of fractions in order to research the anti-cancer activity.The results showed that：A4 components had the best anti-cancer activity,and its IC50 was 0.37mg/mL.According to standard curve of molecular weight,average molecular weight of A4 was about 6723u.关键词
大豆多肽/抗癌活性/凝胶过滤/相对分子量Key words
soybean peptides/anti-cancer activity/gel filtration/relative molecular weight分类
轻工纺织引用本文复制引用
葛锡娟,赵城彬,杨丽丽,吴非..大豆抗癌肽的分离纯化及分子量分布[J].食品工业科技,2012,33(3):99-101,105,4.基金项目
黑龙江省攻关项目 （）
哈尔滨市科技创新人才基金项目 （）
