食品工业科技2012，Vol.33Issue(3)：170-173,236,5.
高产木聚糖酶嗜热子囊菌固体发酵条件与酶学性质
Optimization of xylanase production conditions by Thermoascus aurantiacus QS7-2-4 in solid state fermentation and the properties of xylanase
庞宗文 1郭法谋 1徐勇 1梁静娟 1彭幸 1陆薇1
作者信息
- 1. 广西大学生命科学与技术学院,广西南宁530005
- 折叠
摘要
Abstract
The conditions in solid fermentation of a strain of Thermoascus aurantiacus QS7-2-4 with high yield of xylanase were studied.The research results indicated that the optimum fermentation conditions were：corncob：wheat bran was 7：3（w/w）,the initial pH was 7.2,the water content of medium was 80%,the feeding amount in 250mL flask was 8g,fermentation temperature was 50℃,fermentation time was 72h,the amount of Tween-80 was 0.5%,the best nitrogen source was 1.5% of peptone.The activity of xylanase under the optimum fermentation condition reached 27952U/g.The optimum temperature and pH of this xylanase were 75℃ and 4.5,respectively.The xylanase was stable when the temperature was below 70℃.The activity of xylanase remained 87% after stored for 150d at room temperature.关键词
木聚糖酶/固体发酵/嗜热子囊菌Key words
xylanase/solid state fermentation/Thermoascus aurantiacus分类
轻工纺织引用本文复制引用
庞宗文,郭法谋,徐勇,梁静娟,彭幸,陆薇..高产木聚糖酶嗜热子囊菌固体发酵条件与酶学性质[J].食品工业科技,2012,33(3):170-173,236,5.
