食品工业科技2012，Vol.33Issue(3)：330-333,4.
气相色谱法测定啤酒酵母β-葡聚糖含量
Quantitative analysis of β-glucan from brewers＇yeast by gas chromatography
摘要
Abstract
In order to obtain an accurate content of β-glucan and mannosan prepared from brewers＇yeast,a gas chromatography（GC） method was established through sequential steps of acetylation of hydrolyzed monosaccharide,option of chromatographic column,exploration of temperature program and drawing of standard curve.In addition,corresponding content in the product of Japan ADEKA joint-stock company was compared.The results showed that monosaccharide of β-glucan and mannosan could be separated well after acetylation.The purity of β-glucan and mannosan in product of this experiment reached 32.39% and 16.82%,respectively.However,the corresponding values in Japanese product came up to 30.63% and 16.13%,respectively.Finally,the results of repeatability and recovery experiments indicated that an accurate content of target polysaccharide could be obtained by using the GC method.关键词
啤酒酵母/β-葡聚糖/甘露聚糖/气相色谱法Key words
brewers＇yeast/β-glucan/mannosan/gas chromatography分类
轻工纺织引用本文复制引用
钟超,吴晖,余元善,叶盛权,赖富饶,陈周潭..气相色谱法测定啤酒酵母β-葡聚糖含量[J].食品工业科技,2012,33(3):330-333,4.基金项目
新世纪优秀人才支持计划项目 （）
