食品工业科技2012，Vol.33Issue(3)：337-339,347,4.
直接竞争酶联免疫吸附法检测虾过敏蛋白
Direct competitive enzyme linked immunoassay for detection of shrimp allergic protein
罗奕铭 1王丽 1钟青萍1
作者信息
- 1. 华南农业大学食品学院,广东广州510642
- 折叠
摘要
Abstract
Objective：Direct competitive enzyme linked immunoassay（dcELISA） was established with enzyme-labeled antigen for detecting shrimp allergic protein in foods.Method：The main allergic protein of shrimp was extracted,and immunized mice to prepare polyclonal antibody,horseradish peroxidase（HRP） labeled antigen was also prepared.The dcELISA method was established for detecting shrimp allergic protein.Results：The results indicated that the LOD of the dcELISA was 3.94ng/mL,linear range of the standard curve was 0.12～128.86ng/mL,and the CV of intra-assay and inter-assay were 6.16% and 2.73% respectively.Recovery ranged from 82% to 98%.Conclusion：The method showed better specificity,sensitivity and stability,applying basis for further development of ELISA kit for shrimp allergic protein detection.关键词
虾过敏蛋白/检测/多克隆抗体/酶标抗原/直接竞争酶联免疫吸附法Key words
shrimp allergic protein/detection/polyclonal antibody/enzyme-labeled antigen/direct competitive enzyme linked immunoassay分类
轻工纺织引用本文复制引用
罗奕铭,王丽,钟青萍..直接竞争酶联免疫吸附法检测虾过敏蛋白[J].食品工业科技,2012,33(3):337-339,347,4.
