食品科学2011，Vol.32Issue(16)：31-35,5.
AB-8型大孔吸附树脂分离纯化大叶金花草总黄酮
Using AB-8 Macroporous Adsorption Resin for Separation and Purification of Total Flavonoids from Stenoloma chusana（L.） Ching
摘要
Abstract
Objective： To establish optimal parameters for the separation and purification of total flavonoids from the whole plant of Stenoloma chusana（L.） Ching using AB-8 macroporous adsorption resin.Methods： Static and dynamic adsorption and desorption behaviors of AB-8 macroporous adsorption resin were evaluated for achieving the best purification of total flavonoids.Results： The optimum adsorption and desorption parameters were found to be： sample pH 4.5,sample concentration 1.00 mg/mL,sample loading flow rate 80 mL/h,70% ethanol as the eluent,desorption flow rate 40 mL/h and eluent amount 60 mL,resulting in a total flavonoid purity of 66.16%.Conclusion： AB-8 macroporous adsorption resin is safe,economical and easy to use and can therefore be promisingly applied to separate and purify total flavonoids from the whole plant of Stenoloma chusana（L.） Ching.关键词
大叶金花草/总黄酮/分离纯化Key words
Stenoloma chusana（L.） Ching/total flavonoids/separation and purification分类
化学化工引用本文复制引用
李超,王乃馨,郑义,崔珏,陈华..AB-8型大孔吸附树脂分离纯化大叶金花草总黄酮[J].食品科学,2011,32(16):31-35,5.基金项目
江苏省高校自然科学研究面上项目 （）
徐州工程学院培育项目 （）
