食品科学2011，Vol.32Issue(16)：141-145,5.
超声波-酶解法和超声波法提取裙带菜多糖的比较研究
A Comparative Study of Ultrasonic Treatment Alone and in Combination with Cellulase Hydrolysis for Extraction of Undaria pinnatifida Polysaccharides
张胜帮 1于萍 1曾小明1
作者信息
- 1. 温州大学生命与环境科学学院,浙江温州325035
- 折叠
摘要
Abstract
In the present study,the extraction of Undaria pinnatifida polysaccharides by ultrasonic treatment alone and in combination with cellulase hydrolysis was optimized by orthogonal array designs L16（45） and L9（34）,respectively.The optimal ultrasonic-cellulase treatment conditions for simultaneously improved polysaccharide yield and scavenging activity of polysaccharide extracts against hydroxyl radicals generated in Fenton system were treatment time of 15 min,cellulase amount of 2.8 × 104 U/g algae power,treatment temperature of 70 ℃ and solid-to-liquid ratio of 1：80.Under the optimal extraction conditions,the extraction rate of Undaria pinnatifida polysaccharides was（4.522 ± 0.028）%.The hydroxyl radical scavenging rate of the obtained extract was up to（51.70 ±0.47）%.In addition,each of the four process conditions showed a significant effect on the extraction rate of Undaria pinnatifida polysaccharides（P0.01） and no significant effect on the scavenging activity of polysaccharide extracts against hydroxyl radicals（P0.05）.The combination of ultrasonic and cellulase could significantly increase the extraction rate of Undaria pinnatifida polysaccharides and the scavenging activity of polysaccharide extracts against hydroxyl radicals as compared with ultrasonic alone（P0.01）.关键词
超声波-酶解法/超声提取/裙带菜/多糖/羟自由基Key words
ultrasonic-enzymatic extraction/ultrasonic extraction/Undaria pinnatifida/polysaccharide/hydroxyl radical分类
轻工纺织引用本文复制引用
张胜帮,于萍,曾小明..超声波-酶解法和超声波法提取裙带菜多糖的比较研究[J].食品科学,2011,32(16):141-145,5.
