食品科学2011，Vol.32Issue(16)：236-239,4.
食品中3种致病菌多重PCR检测体系的建立及初步应用
Establishment and Application of a Multiplex PCR Assay for Detection of Three Pathogenic Bacteria in Food
摘要
Abstract
Objective： To develop a rapid multiplex polymerase chain reaction（m-PCR） assay for simultaneous detection of Salmonella spp.,Staphylococcus aureus and Listeria monocytogenes in food.Methods： The genomic alignment method was used to identify specific PCR target sequences.Three pairs of specific primers were designed with Primer Premier 5.0 according to the invasive protein gene（invA） of Salmonella,the nuc gene of Staphylococcus aureus and the prs gene of Listeria monocytogen.Multiplex PCR was established by optimizing the reaction system.Results： The sensitivity of the multiplex PCR method was 7.6 pg/μL for Salmonellla spp.,3.8 pg/μL for Staphylococcus aureus,and 5.1 pg/μL for Listeria monocytogenes.All specific primers were amplified,and their corresponding strips were observed in validation experiments without cross reactivity.Conclusions： A triple PCR assay has been established for the simultaneous,sample,rapid and sensitive detection of Salmonella spp.,Staphylococcus aureus and Listeria monocytogenes in food.关键词
食源性致病菌/多重聚合酶链式反应（PCR）/沙门氏菌/金黄色葡萄球菌/单核细胞增生性李斯特菌/食品检测Key words
food-borne pathogenic bacteria/multiplex polymerase chain reaction（m-PCR）/Salmonella spp./Staphylococcus aureus/Listeria monocytogen/food inspection分类
轻工纺织引用本文复制引用
钱志伟,孙新城..食品中3种致病菌多重PCR检测体系的建立及初步应用[J].食品科学,2011,32(16):236-239,4.基金项目
河南省科技厅重点攻关项目 （）
